Johne’s direct fecal PCR plus Johne’s commercial ELISA on all animals-the most aggressive and costly method. Herd testing options, from highest to lowest quality of information and cost, are as follows: Johne’s direct fecal PCR, Johne’s culture, pooled fecal culture, and the commercial ELISA tests can be used in various combinations, with the most information for detecting MAP infection coming at the greatest cost. Testing is most useful at the herd-and not the individual animal-level. Selection of Tests for Bovine Herd Testing For this reason, a combination of fecal detection testing by PCR and serologic testing by ELISA is typically recommended for management strategies in cow/calf herds. It should also be noted that there is a subset of animals that may be negative on this ELISA but still shedding MAP. Confirmation of seroreactors by PCR is strongly recommended.
For this reason, serological testing is considered a presumptive test at the herd or individual animal level. Testing is typically not recommended for cattle 99%, false-positive results can occur from exposure to other cross-reacting environmental mycobacteria. The laboratory participates in an annual proficiency test for Johne’s serology, cultures, and PCR tests and is approved to do Johne’s testing for the USDA Voluntary Johne’s Control Program.īreakdown of sample and test options based on heard species
Johne’s disease, or Pseudotuberculosis is caused by the bacterium, Mycobacterium avium subsp paratuberculosis (MAP).
Caprine form full#
Out-of-State Johne’s testing is offered at the full fee please refer to the Animal Health Diagnostic Center’s Test & Fee Schedule listings for specifics on individual tests. An additional testing subsidy is provided to herds enrolled in the NYSCHAP Johne’s Module. The Johne’s Program in New York State (NYS) is administered through the NYS Cattle Health Assurance Program (NYSCHAP). The NYS Department of Agriculture & Markets provides Johne’s testing subsidies for all cattle herds in New York State.
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